TREK1/KCNK2 Polyclonal antibody

TREK1/KCNK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26807-1-AP

Synonyms

hTREK 1c, hTREK 1e, K2p2.1, KCNK2, TPKC1, TREK, TREK 1, TREK 1 K(+) channel subunit, TREK1

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Validation Data Gallery

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  WB analysis using 26807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26807-1-AP (TREK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26807-1-AP targets TREK1/KCNK2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TREK1/KCNK2 fusion protein Ag25209
• Full Name: potassium channel, subfamily K, member 2
• Calculated Molecular Weight: 411 aa, 46 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC101693
• Gene Symbol: KCNK2
• Gene ID (NCBI): 3776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tandem of pore domains in a weak inward rectifying K+ channel (TWIK1, K2P1.1, or KCNK1) and TWIK-related K+channel 1 (TREK1, K2P 2.1, or KCNK2) are members of the two pore domain potassium (K2P) channel family, consisting of 15 channels that regulate the stabilization of resting membrane potential and cellular excitability by wielding background K+ leakage currents (PMID:12580339). TREK1/KCNK2 is sensitive to a wide range of physical and chemical cues. Its main role is to maintain the resting potential of the cell and whilst highly expressed in the nervous system, TREK1/KCNK2 is also expressed in the kidney, heart, lung and smooth muscle cells (PMID:31031627).