TIMD4 Polyclonal antibody

TIMD4 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12008-1-AP

Synonyms

SMUCKLER, T cell membrane protein 4, TIM 4, TIM4, TIMD 4, TIMD4

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Validation Data Gallery

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  IHC staining of human tonsillitis using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIMD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12008-1-AP (TIMD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIMD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12008-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12008-1-AP (TIMD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 12008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIMD4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 12008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TIMD4 antibody (12008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

12008-1-AP targets TIMD4 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMD4 fusion protein Ag2621
• Full Name: T-cell immunoglobulin and mucin domain containing 4
• Calculated Molecular Weight: 378 aa, 42 kDa
• GenBank Accession Number: BC008988
• Gene Symbol: TIMD4
• Gene ID (NCBI): 91937
• RRID: AB_2877816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
goat		Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
human	IHC	Front Oncol; Single-Cell RNA-seq Reveals Characteristics of Malignant Cells and Immune Microenvironment in Subcutaneous Panniculitis-Like T-Cell Lymphoma.; Authors - Zifeng Li
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
mouse	IF	Liver Int; Regulation of the macrophage-hepatic stellate cell interaction by targeting macrophage PPARγ to prevent NASH progression in mice; Authors - Xi-Xi Ni