TERF2IP Polyclonal antibody

TERF2IP Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14595-1-AP

Synonyms

DRIP5, hRap1, RAP1 homolog, TERF2IP

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Validation Data Gallery

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  WB analysis of HEK-293 using 14595-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14595-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14595-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14595-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14595-1-AP

  IP result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug.

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14595-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 14595-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TERF2IP antibody (14595-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, HT-1080 cells, human placenta tissue, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14595-1-AP targets TERF2IP in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TERF2IP fusion protein Ag6130
• Full Name: telomeric repeat binding factor 2, interacting protein
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005841
• Gene Symbol: TERF2IP
• Gene ID (NCBI): 54386
• RRID: AB_10642697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Preferential extension of short telomeres induced by low extracellular pH.; Authors - Yuanlong Ge
human	WB	Exp Cell Res; C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.; Authors - Radhika Koranne