TERF1 Monoclonal antibody

TERF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D10G4

Cat no : 67592-1-Ig

Synonyms

FLJ41416, hTRF1 AS, NIMA interacting protein 2, PIN2, t TRF1, Telomeric protein Pin2/TRF1, TERF1, TRBF1, TRF, TRF1, TTAGGG repeat binding factor 1

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Validation Data Gallery

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  WB analysis of K-562 using 67592-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67592-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67592-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67592-1-Ig (TERF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67592-1-Ig targets TERF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TERF1 fusion protein Ag12540
• Full Name: telomeric repeat binding factor (NIMA-interacting) 1
• Calculated Molecular Weight: 439 aa, 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC029378
• Gene Symbol: TERF1
• Gene ID (NCBI): 7013
• RRID: AB_2918494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Telomeres are nucleoprotein complexes located at chromosome ends that consist of tandem arrays of TTAGGG repeats bound to specific proteins. Telomeres protect chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. TERF1 (telomeric repeat-binding factor 1) binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. It's one component of the shelterin complex, which involved in the regulation of telomere length and protection.