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CoraLite® Plus 488-conjugated TBC1D4 Monoclonal antibody

TBC1D4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2H2

Cat no : CL488-68063

Synonyms

Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TBC1D4 Monoclonal an (CL488-68063)

    IF Staining of HeLa using CL488-68063

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 TBC1D4 antibody (CL488-68063, Clone: 1E2H2 ) at dilution of 1:500.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68063 targets TBC1D4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TBC1D4 fusion protein Ag20341
Full Name TBC1 domain family, member 4
Calculated Molecular Weight 1299 aa, 147 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC151239
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TBC1D4 antibody CL488-68063Download protocol
Standard Protocols
Click here to view our Standard Protocols