CoraLite® Plus 488-conjugated SYK Monoclonal antibody

• Featured Product
• KD/KO Validated

SYK Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C4A12

Cat no : CL488-66721

Synonyms

DKFZp313N1010, FLJ25043, FLJ37489, spleen tyrosine kinase, SYK, Tyrosine protein kinase SYK

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Validation Data Gallery

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  WB analysis using CL488-66721

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66721 (SYK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66721 targets SYK in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SYK fusion protein Ag17654
• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 635 aa, 72 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing.