STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B5H2

Cat no : 68155-1-Ig

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

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Validation Data Gallery

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  WB analysis using 68155-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IP experiment of Jurkat using 68155-1-Ig

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with Jurkat cells lysate 2400 ug.

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  IP experiment of HeLa using 68155-1-Ig

  IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of mouse liver using 68155-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68155-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68155-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68155-1-Ig targets STIP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STIP1 fusion protein Ag7851
• Full Name: stress-induced-phosphoprotein 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC002987
• Gene Symbol: STIP1
• Gene ID (NCBI): 10963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).