SPAG8 Polyclonal antibody

SPAG8 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13915-1-AP

Synonyms

BS 84, HSD 1, hSMP 1, SMP 1, SMP1, SPAG3, SPAG8, sperm associated antigen 8, Sperm membrane protein 1, Sperm membrane protein BS 84

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Validation Data Gallery

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  WB analysis using 13915-1-AP

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13915-1-AP

  IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug.

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  IHC staining of human prostate cancer using 13915-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 13915-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of PC-3 using 13915-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13915-1-AP (SPAG8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13915-1-AP targets SPAG8 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG8 fusion protein Ag4910
• Full Name: sperm associated antigen 8
• Calculated Molecular Weight: 45 kDa, 54 kDa
• Observed Molecular Weight: 56-70 kDa
• GenBank Accession Number: BC019247
• Gene Symbol: SPAG8
• Gene ID (NCBI): 26206
• RRID: AB_2194804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.

Publications

Species	Application	Title
mouse	WB	Development; Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice.; Authors - Gina Esther Merges
human	IHC	J Immunother; Sperm-associated antigens as targets for cancer immunotherapy: expression pattern and humoral immune response in cancer patients.; Authors - Sili?a Karīna K