SORD Monoclonal antibody

SORD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4A8E1

Cat no : 67625-1-Ig

Synonyms

L iditol 2 dehydrogenase, SDH, sorbitol dehydrogenase, SORD, SORD1

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Validation Data Gallery

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  WB analysis using 67625-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of HSC-T6 using 67625-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67625-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67625-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Hela cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

67625-1-Ig targets SORD in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SORD fusion protein Ag8651
• Full Name: sorbitol dehydrogenase
• Calculated Molecular Weight: 357 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC021085
• Gene Symbol: SORD
• Gene ID (NCBI): 6652
• RRID: AB_2882826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publications

Species	Application	Title
mouse	WB	J Nutr; Sucrose intake elevates erythritol in plasma and urine in male mice; Authors - Semira R Ortiz ; KO Validated
human	WB	Front Nutr; Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells; Authors - Semira R. Ortiz ; KD Validated