SNAPC5 Polyclonal antibody

SNAPC5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17272-1-AP

Synonyms

SNAP19, SNAPc 19 kDa subunit, SNAPc subunit 5, SNAPC5

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Validation Data Gallery

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  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17272-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 17272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 17272-1-AP

  Immunofluorescent analysis of HeLa cells using 17272-1-AP (SNAPC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 17272-1-AP is SNAPC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAPC5 fusion protein Ag11029
• Full Name: small nuclear RNA activating complex, polypeptide 5, 19kDa
• Calculated Molecular Weight: 68aa,8 kDa; 98aa,11 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC014315
• Gene Symbol: SNAPC5
• Gene ID (NCBI): 10302
• RRID: AB_2878373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAPC5, also named as SNAP19, is snRNA-activating protein complex 19 kDa. It's a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes.

Publications

Species	Application	Title
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang