SMPD1,ASM Polyclonal antibody

• Featured Product
• KD/KO Validated

SMPD1,ASM Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 14609-1-AP

Synonyms

ASM, SMPD1, SMPD1,ASM

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Validation Data Gallery

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  WB analysis using 14609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14609-1-AP (SMPD1,ASM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14609-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14609-1-AP (SMPD1,ASM Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: human brain tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

14609-1-AP targets SMPD1,ASM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMPD1,ASM fusion protein Ag6180
• Full Name: sphingomyelin phosphodiesterase 1, acid lysosomal
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 60 kDa, 70-75 kDa
• GenBank Accession Number: BC041164
• Gene Symbol: SMPD1
• Gene ID (NCBI): 6609
• RRID: AB_2878068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing.

Publications

Species	Application	Title
mouse		Cell Host Microbe; The β2Integrin Mac-1 Induces Protective LC3-Associated Phagocytosis of Listeria monocytogenes.; Authors - Alexander Gluschko ; KO Validated
human	WB,IF	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
mouse	IF	Acta Neuropathol Commun; Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse; Authors - Simone M Crivelli
mouse	IF	Cells; Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease; Authors - Ahmed Elsherbini
human	IHC	J Mol Med (Berl); Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma; Authors - Gregory C Wilson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alice (Verified Customer) (05-11-2021)

Good Ab should show good result in your website to convince people to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Pancreas

SMPD1,ASM Antibody Immunohistochemistry validation (1:200 dilution) in Mouse Pancreas (Cat no:14609-1-AP)