SMARCD3 Polyclonal antibody

SMARCD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12838-1-AP

Synonyms

BAF60C, BRG1 associated factor 60C, CRACD3, Rsc6p, SMARCD3

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Validation Data Gallery

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  WB analysis using 12838-1-AP

  C6 and NIH3T3 cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12838-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12838-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, BxPC-3 cells, HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human heart tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

12838-1-AP targets SMARCD3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCD3 fusion protein Ag3896
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-55 kDa
• GenBank Accession Number: BC002628
• Gene Symbol: SMARCD3
• Gene ID (NCBI): 6604
• RRID: AB_2192157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCD3 is a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex, which evolutionarily conserved from yeast to human, and all complexes contain a core set of conserved components, including BRG1/Brm-associated factors (BAFs) and a DNA-dependent SWI2/SNF2-like ATPase, which enables chromatin remodeling. SMARCD3 isoforms bind to several nuclear receptors and transcription factors of various families. SMARCD3 proteins interact in a ligand-independent manner with peroxisome proliferator- activated receptor gamma and enhance its transcriptional activity.

Publications

Species	Application	Title
human	WB	Cell Discov; m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.; Authors - Wei Zhuo
mouse	WB	Cardiovasc Res; The G protein coupled receptor Apj is expressed in the second heart field and regulates Cerberus-Baf60C axis in embryonic stem cell cardiomyogenesis.; Authors - D'Aniello Cristina C
human	WB	Transl Oncol; SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness.; Authors - Ying Chen
human	WB	Cells; The SMARCD Family of SWI/SNF Accessory Proteins Is Involved in the Transcriptional Regulation of Androgen Receptor-Driven Genes and Plays a Role in Various Essential Processes of Prostate Cancer; Authors - Iris E Ertl