CAT-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAT-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 14195-1-AP

Synonyms

Amino acid transporter cationic 1, ATRC1, CAT 1, CAT1, CAT-1, HCAT1, REC1L, SLC7A1

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Validation Data Gallery

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  WB analysis of rat brain/liver tissue using 14195-1-AP

  WB result of 14195-1-AP from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.

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  WB analysis using 14195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14195-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14195-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human osteosarcoma using 14195-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, Jurkat cells, HepG2 cells, rat brain/liver tissue, mouse heart tissue, rat liver tissue
• Positive IHC detected in: human osteosarcoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

14195-1-AP targets CAT-1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAT-1 fusion protein Ag5398
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC063303
• Gene Symbol: SLC7A1
• Gene ID (NCBI): 6541
• RRID: AB_2190723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell; In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.; Authors - Hongling Huang ; KO Validated
mouse	IHC	J Cachexia Sarcopenia Muscle; Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.; Authors - Thomas Kunzke
mouse, human	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes
human	IHC	Front Oncol; An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.; Authors - Zhuo Lin
rat	IHC	Exp Neurol; Pseudotype-dependent lentiviral transduction of astrocytes or neurons in the rat substantia nigra.; Authors - Cannon Jason R JR