NPT1 Polyclonal antibody

NPT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20751-1-AP

Synonyms

Na(+)/PI cotransporter 1, Na/Pi 4, NAPI 1, SLC17A1

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Validation Data Gallery

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  WB analysis of human placenta using 20751-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20751-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 20751-1-AP is NPT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPT1 fusion protein Ag12819
• Full Name: solute carrier family 17 (sodium phosphate), member 1
• Calculated Molecular Weight: 467 aa, 51 kDa
• Observed Molecular Weight: 50 kDa, 60-64 kDa
• GenBank Accession Number: BC101745
• Gene Symbol: SLC17A1
• Gene ID (NCBI): 6568
• RRID: AB_10693677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPT1 (also known as SLC17A1) is a member of sodium-dependent inorganic phosphate transporters (NPT), which regulates entrance into the cellular membrane. NPT1 is mainly expressed in the kidney transporting small organic anions such as PAH (para-aminohippurate), but it is also found in the liver and brain. NTP1 localizes to the apical membrane of renal proximal tubular cells. NPT1 exits two isoforms due to the alternative splicing. Western blot analysis using this antibody detected a major band around 45-55 kDa in kidney tissue.

Publications

Species	Application	Title
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
mouse	WB	Phytomedicine; Eurycomanol alleviates hyperuricemia by promoting uric acid excretion and reducing purine synthesis.; Authors - Ruixia Bao
mouse	WB	Front Pharmacol; Effect of Eurycoma longifolia Stem Extract on Uric Acid Excretion in Hyperuricemia Mice.; Authors - Ruixia Bao