SLC13A4 Polyclonal antibody

SLC13A4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20302-1-AP

Synonyms

NaS2, SLC13A4, SUT 1, SUT1

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 20302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20302-1-AP (SLC13A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20302-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20302-1-AP (SLC13A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 20302-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20302-1-AP (SLC13A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 20302-1-AP is SLC13A4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC13A4 fusion protein Ag14116
• Full Name: solute carrier family 13 (sodium/sulfate symporters), member 4
• Calculated Molecular Weight: 626 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC030689
• Gene Symbol: SLC13A4
• Gene ID (NCBI): 26266
• RRID: AB_10793722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC13A4 (also known as SUT-1 or NaS2) belongs to the family of Na+-coupled anion transporters and mediates sulfate reabsorption in the high endothelial venules (HEV). Sulfate is an obligate nutrient for fetal development. Highly expressed in placenta, SLC13A4 has been proposed to play important physiological roles in maintaining high maternal serum sulfate levels during pregnancy and mediating sulfate supply to the fetus.

Publications

Species	Application	Title
mouse	WB,IHC	Biol Reprod; Placental, renal, and ileal sulfate transporter gene expression in mouse gestation.; Authors - Dawson Paul A PA
mouse	IHC	EBioMedicine; Postnatal N-acetylcysteine administration rescues impaired social behaviors and neurogenesis in Slc13a4 haploinsufficient mice.; Authors - Zhe Zhang