SLC10A3 Polyclonal antibody

SLC10A3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19909-1-AP

Synonyms

DXS253E, P3, P3 protein, SLC10A3

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Validation Data Gallery

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  WB analysis using 19909-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19909-1-AP (SLC10A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 19909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19909-1-AP (SLC10A3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 19909-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19909-1-AP (SLC10A3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19909-1-AP

  Immunofluorescent analysis of Hela cells, using SLC10A3 antibody 19909-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: 37°C incubated A549 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19909-1-AP targets SLC10A3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC10A3 fusion protein Ag13915
• Full Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 3
• Calculated Molecular Weight: 477 aa, 50 kDa
• Observed Molecular Weight: 50 kDa, 48 kDa
• GenBank Accession Number: BC004966
• Gene Symbol: SLC10A3
• Gene ID (NCBI): 8273
• RRID: AB_10642950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The solute carrier (SLC) family 10 (SLC10) encodes protein for transporters of various agents, bile acids and steroidal hormones, and SLC01A3 belongs to the SLC10 family. SLC10A3 consists of 477 amino acids, with a calculated mass of approximately 50 kDa.