CoraLite® Plus 488-conjugated SH3GLB2 Monoclonal antibody

SH3GLB2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B1A10

Cat no : CL488-68325

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68325

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SH3GLB2 antibody (CL488-68325, Clone: 4B1A10 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68325 targets SH3GLB2 in IF applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SH3GLB2 fusion protein Ag8932
• Full Name: SH3-domain GRB2-like endophilin B2
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC014635
• Gene Symbol: SH3GLB2
• Gene ID (NCBI): 56904
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).