SH3BP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SH3BP1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20541-1-AP

Synonyms

3BP 1, SH3 domain binding protein 1, SH3BP1

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Validation Data Gallery

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  WB analysis using 20541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20541-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20541-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20541-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20541-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20541-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20541-1-AP (SH3BP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 20541-1-AP

  Immunofluorescent analysis of HepG2 cells, using SH3BP1 antibody 20541-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20541-1-AP

  Immunofluorescent analysis of Hela cells, using SH3BP1 antibody 20541-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, mouse lung tissue, mouse spleen tissue
• Positive IF detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

20541-1-AP targets SH3BP1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3BP1 fusion protein Ag14575
• Full Name: SH3-domain binding protein 1
• Calculated Molecular Weight: 701 aa, 76 kDa
• Observed Molecular Weight: 76-80 kDa, 51 kDa
• GenBank Accession Number: BC008282
• Gene Symbol: SH3BP1
• Gene ID (NCBI): 23616
• RRID: AB_10694570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.; Authors - Daniel Schlam
human	WB	Cancer Med; Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia.; Authors - Kibeom Park ; KD Validated