SGLT3/SLC5A4 Polyclonal antibody

SGLT3/SLC5A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24327-1-AP

Synonyms

Na(+)/glucose cotransporter 3, SAAT1, SGLT3, SGLT3/SLC5A4, SLC5A4, Sodium/glucose cotransporter 3

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Validation Data Gallery

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  WB analysis of mouse kidney using 24327-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 24327-1-AP

  IP result of anti-SGLT3/SLC5A4 (IP:24327-1-AP, 4ug; Detection:24327-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 24327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50.

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  IHC staining of human kidney using 24327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

24327-1-AP targets SGLT3/SLC5A4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGLT3/SLC5A4 fusion protein Ag19391
• Full Name: solute carrier family 5 (low affinity glucose cotransporter), member 4
• Calculated Molecular Weight: 659 aa, 72 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC153069
• Gene Symbol: SLC5A4
• Gene ID (NCBI): 6527
• RRID: AB_2650519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC5A4, also known as SGLT3, is a sugar sensor that depolarizes the plasma membrane in response to external glucose (PMID: 12748858, 20421923). SGLT3 mediates the influx of sodium ions into the cell but does not transport sugars, also potently activated by imino sugars such as deoxynojirimycin (PMID: 22766068, 13130073). SGLT3 is a plasma membrane protein, shares 70% identity with SGLT1, and is expressed in the small intestine (cholinergic neurons), skeletal muscle, kidney, uterus, and testis (PMID: 12748858). SGLT3 protein at apparent molecular masses of 72 kDa and 80 kDa in human kidney tissue and HK-2 cells (PMID: 22766068).

Publications

Species	Application	Title
mouse	WB,IF	Int J Environ Res Public Health; Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice.; Authors - Caterina Franco
human	WB,IF	Life Sci; Intestinal sodium/glucose cotransporter 3 expression is epithelial and downregulated in obesity.; Authors - Matúš Soták
human	IHC	PLoS One; Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment.; Authors - Emi Ono