SFXN1 Monoclonal antibody

SFXN1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D9F1

Cat no : 68024-1-Ig

Synonyms

FLJ12876, SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

---

Validation Data Gallery

• 

  WB analysis using 68024-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IF Staining of MCF-7 using 68024-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: PC-3 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, Rat brain tissue, Mouse brain tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68024-1-Ig targets SFXN1 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SFXN1 fusion protein Ag2985
• Full Name: sideroflexin 1
• Calculated Molecular Weight: 35.6 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC020517
• Gene Symbol: SFXN1
• Gene ID (NCBI): 94081
• RRID: AB_2918768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.