SERPINA10 Polyclonal antibody

SERPINA10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11851-1-AP

Synonyms

PZI, Serpin A10, SERPINA10, ZPI

---

Validation Data Gallery

• 

  WB analysis of human liver using 11851-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11851-1-AP

  IP result of anti-SERPINA10 (IP:11851-1-AP, 3ug; Detection:11851-1-AP 1:1000) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 11851-1-AP

  Immunofluorescent analysis of L02 cells using 11851-1-AP (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11851-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINA10 antibody 11851-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: human liver tissue, HepG2 cells, L02 cells, mouse liver tissue, RAW264.7
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: L02 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11851-1-AP targets SERPINA10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SERPINA10 fusion protein Ag2420
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• Calculated Molecular Weight: 444 aa, 51 kDa
• Observed Molecular Weight: 72 kDa, 68 kDa, 50-55 kDa
• GenBank Accession Number: BC022261
• Gene Symbol: SERPINA10
• Gene ID (NCBI): 51156
• RRID: AB_2254343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.