RanBP1 Polyclonal antibody

RanBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27804-1-AP

Synonyms

HTF9A, RAN binding protein 1, RANBP1

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Validation Data Gallery

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  WB analysis using 27804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27804-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 27804-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 27804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HEK-293 cells, mouse brain tissue, fetal human brain tissue, HeLa cells, RAW 264.7 cells, NIH/3T3 cells, 3T3-L1 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

27804-1-AP targets RanBP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RanBP1 fusion protein Ag25714
• Full Name: RAN binding protein 1
• Observed Molecular Weight: 28 kDa
• Gene Symbol: RANBP1
• Gene ID (NCBI): 5902
• RRID: AB_2880977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

Publications

Species	Application	Title
human	IF	J Med Chem; Structure-Guided Design of the First Noncovalent Small-Molecule Inhibitor of CRM1.; Authors - Yuqin Lei
human	WB	Elife; Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.; Authors - Yuling Li