RPS21 Polyclonal antibody

• Featured Product
• KD/KO Validated

RPS21 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16946-1-AP

Synonyms

40S ribosomal protein S21, ribosomal protein S21, RPS21

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Validation Data Gallery

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  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 16946-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16946-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16946-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16946-1-AP (RPS21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, HepG2 cells, SGC-7901 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

16946-1-AP targets RPS21 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPS21 fusion protein Ag10557
• Full Name: ribosomal protein S21
• Calculated Molecular Weight: 81 aa, 9 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC018140
• Gene Symbol: RPS21
• Gene ID (NCBI): 6227
• RRID: AB_2180351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Publications

Species	Application	Title
human	IHC	Oncol Lett; Identification of the key genes and pathways in prostate cancer.; Authors - Shutong Fan
human	WB,IHC	Med Oncol; Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.; Authors - Zumu Liang ; KD Validated
mouse	IHC	Reprod Sci; Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes; Authors - Natsuki Nakanishi