RPL29 Polyclonal antibody

RPL29 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (2)

Cat no : 15799-1-AP

Synonyms

60S ribosomal protein L29, HIP, HUMRPL29, ribosomal protein L29, RPL29

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Validation Data Gallery

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 15799-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, NIH/3T3 cells, C6 cells, mouse pancreas tissue, HUVEC cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15799-1-AP targets RPL29 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL29 fusion protein Ag8510
• Full Name: ribosomal protein L29
• Calculated Molecular Weight: 159 aa, 18 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC008926
• Gene Symbol: RPL29
• Gene ID (NCBI): 6159
• RRID: AB_2878187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human,mouse	WB	Sci Rep; The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29.; Authors - Mohammad Ishaque Ali
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A great antibody with strong signal and specificity. We confirmed this antibody in HCT116 and HEK293T cell lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

FH

H (Verified Customer) (04-05-2020)

This antibody works very well. I used this antibody when screening clones for CRISPR knock-in.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPL29 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:15799-1-AP)