RPL10L Polyclonal antibody

RPL10L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16222-1-AP

Synonyms

60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L

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Validation Data Gallery

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  WB analysis using 16222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, SKOV-3 cells, mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16222-1-AP targets RPL10L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL10L fusion protein Ag9275
• Full Name: ribosomal protein L10-like
• Calculated Molecular Weight: 214 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC014310
• Gene Symbol: RPL10L
• Gene ID (NCBI): 140801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).