CoraLite® Plus 488-conjugated RGS4 Monoclonal antibody

RGS4 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E2E2

Cat no : CL488-66441

Synonyms

DKFZp761F1924, RGP4, RGS4, SCZD9

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Validation Data Gallery

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  WB analysis using CL488-66441

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-66441

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66441 (RGS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C6 cells, Neuro-2a cells, Raji cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66441 targets RGS4 in WB applications and shows reactivity with human, rat, mouse, pig samples.

• Tested Reactivity: human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RGS4 fusion protein Ag6029
• Full Name: regulator of G-protein signaling 4
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC051869
• Gene Symbol: RGS4
• Gene ID (NCBI): 5999
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.