RFX6 Polyclonal antibody

RFX6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22551-1-AP

Synonyms

DNA binding protein RFX6, Regulatory factor X 6, regulatory factor X, 6, RFX6, RFXDC1

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Validation Data Gallery

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  WB analysis of Caco-2 using 22551-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 22551-1-AP (RFX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 22551-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 22551-1-AP (RFX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 22551-1-AP is RFX6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFX6 fusion protein Ag18235
• Full Name: regulatory factor X, 6
• Calculated Molecular Weight: 928 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC039248
• Gene Symbol: RFX6
• Gene ID (NCBI): 222546
• RRID: AB_2879120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX6, also known as RFXDC1, is a 928 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. RFX6 is specifically expressed in pancreas, small intestine and colon. Mutations in the gene encoding RFX6 is the cause of the Mitchell-Riley syndrome (MIRIS), which is characterized by neonatal diabetes, duodenal and jejunal atresia, a hypoplastic or annular pancreas and absent gallbladder.

Publications

Species	Application	Title
human	WB,IHC	Clin Transl Med; RFX6 facilitates aerobic glycolysis-mediated growth and metastasis of hepatocellular carcinoma through targeting PGAM1; Authors - Zhiyu Qiu ; KD Validated