RBM22 Polyclonal antibody

RBM22 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 22103-1-AP

Synonyms

fSAP47, Pre mRNA splicing factor RBM22, RBM22, RNA binding motif protein 22, ZC3H16

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Validation Data Gallery

• 

  WB analysis of Raji using 22103-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22103-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22103-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22103-1-AP targets RBM22 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM22 fusion protein Ag17315
• Full Name: RNA binding motif protein 22
• Calculated Molecular Weight: 420 aa, 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC003402
• Gene Symbol: RBM22
• Gene ID (NCBI): 55696
• RRID: AB_2878996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000