RBM19 Polyclonal antibody

RBM19 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19095-1-AP

Synonyms

KIAA0682, RBM19, RNA binding motif protein 19

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Validation Data Gallery

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  WB analysis of mouse colon using 19095-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 19095-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 19095-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19095-1-AP (RBM19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 19095-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19095-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19095-1-AP (RBM19 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 19095-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RBM19 antibody 19095-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse colon tissue, COLO 320 cells, mouse small intestine tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19095-1-AP is RBM19 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM19 fusion protein Ag13545
• Full Name: RNA binding motif protein 19
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC006137
• Gene Symbol: RBM19
• Gene ID (NCBI): 9904
• RRID: AB_10640906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM19, also named as KIAA0682, is a 960 amino acid protein, which belongs to the RRM MRD1 family. RBM19 is expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia. RBM19 plays a role in embryo pre-implantation development. The calculated molecular weight of RBM19 is 107 kDa, but the modified RBM19 is about 120 kDa.