RBM17 Polyclonal antibody

RBM17 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13918-1-AP

Synonyms

45 kDa splicing factor, RBM17, RNA binding motif protein 17, SPF45, Splicing factor 45

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Validation Data Gallery

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  WB analysis using 13918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13918-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13918-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13918-1-AP (RBM17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 13918-1-AP

  Immunofluorescent analysis of Hela cells, using RBM17 antibody 13918-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, MCF-7 cells, HeLa cells, A2780 cells, PC-3 cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13918-1-AP targets RBM17 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM17 fusion protein Ag4921
• Full Name: RNA binding motif protein 17
• Calculated Molecular Weight: 401 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC039322
• Gene Symbol: RBM17
• Gene ID (NCBI): 84991
• RRID: AB_2238079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	J Exp Clin Cancer Res; CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.; Authors - Dongni Chen