RABL3 Monoclonal antibody

RABL3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H3E1

Cat no : 68342-1-Ig

Synonyms

Rab like protein 3, RABL3

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Validation Data Gallery

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  WB analysis using 68342-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68342-1-Ig (RABL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IF Staining of A549 using 68342-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABL3 antibody (68342-1-Ig, Clone: 2H3E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68342-1-Ig targets RABL3 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RABL3 fusion protein Ag33137
• Full Name: RAB, member of RAS oncogene family-like 3
• Calculated Molecular Weight: 236 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC020832
• Gene Symbol: RABL3
• Gene ID (NCBI): 285282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).