PSMG3 Polyclonal antibody

PSMG3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15601-1-AP

Synonyms

C7orf48, hPAC3, PAC 3, PAC3, PSMG3

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Validation Data Gallery

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  WB analysis of HeLa using 15601-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15601-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15601-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15601-1-AP (PSMG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15601-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15601-1-AP (PSMG3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15601-1-AP targets PSMG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMG3 fusion protein Ag7984
• Full Name: proteasome (prosome, macropain) assembly chaperone 3
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13-18 kDa
• GenBank Accession Number: BC004308
• Gene Symbol: PSMG3
• Gene ID (NCBI): 84262
• RRID: AB_2878156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMG3, also named as C7orf48 and PAC3, is a chaperone protein which promotes assembly of the 20S proteasome. It may cooperate with PSMG1-PSMG2 heterodimers to orchestrate the correct assembly of proteasomes.