PSMB6 Polyclonal antibody

PSMB6 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11684-2-AP

Synonyms

20S proteasome, DELTA, LMPY, Macropain delta chain, Proteasome delta chain, Proteasome subunit beta type 6, Proteasome subunit Y, PSMB6

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Validation Data Gallery

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  WB analysis using 11684-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11684-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11684-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11684-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 11684-2-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 11684-2-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HeLa using 11684-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11684-2-AP targets PSMB6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB6 fusion protein Ag2296
• Full Name: proteasome (prosome, macropain) subunit, beta type, 6
• Calculated Molecular Weight: 239 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000835
• Gene Symbol: PSMB6
• Gene ID (NCBI): 5694
• RRID: AB_2172199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

Publications

Species	Application	Title
rat	WB	Cell Physiol Biochem; The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.; Authors - Wenjin Guo
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu