PSMB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMB2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15154-1-AP

Synonyms

20S proteasome, HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

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Validation Data Gallery

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  WB analysis using 15154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  WB result of PSMB2 antibody (15154-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

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  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15154-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15154-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15154-1-AP

  Immunofluorescent analysis of HepG2 cells using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

15154-1-AP targets PSMB2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB2 fusion protein Ag7198
• Full Name: proteasome (prosome, macropain) subunit, beta type, 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000268
• Gene Symbol: PSMB2
• Gene ID (NCBI): 5690
• RRID: AB_2300322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4(PMID: 7918633). The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions (PMID: 21190828). Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion (PMID: 29780166, PMID: 36110152).

Publications

Species	Application	Title
human	WB	Oncogene; NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.; Authors - Sheng Tan ; KD Validated
human	WB	Mol Cancer Res; Proteasome subunits differentially control Myeloma cell viability and proteasome inhibitor sensitivity.; Authors - Chang-Xin Shi
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
rat	WB	Metab Brain Dis; Molecular alteration of the proteasome contributes to AD-like pathology in the brain of HFD-STZ diabetic rats; Authors - Han Gao
rat	WB	Cell Mol Neurobiol; Inhibition of the Proteasome Regulator PA28 Aggravates Oxidized Protein Overload in the Diabetic Rat Brain; Authors - Dong-Gui Wu
mouse	WB	PLoS Biol; Pharmacological induction of autophagy reduces inflammation in macrophages by degrading immunoproteasome subunits; Authors - Jiao Zhou