• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PRY Polyclonal antibody

PRY Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24688-1-AP

Synonyms

PRY, PRY2, PRYP3, PRYP4, PTPN13 like protein, Y linked, PTPN13 like, Y linked 2, PTPN13LY, PTPN13LY2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of PC-3 cells using PRY Polyclonal antibody (24688-1-AP)

    WB analysis of PC-3 using 24688-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24688-1-AP (PRY antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRY Polyclonal antibody (24688-1-AP)

    IF Staining of HepG2 using 24688-1-AP

    Immunofluorescent analysis of HepG2 cells using 24688-1-AP (PRY antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24688-1-AP is PRY Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRY fusion protein Ag20232
Full Name PTPN13-like, Y-linked 2
Calculated Molecular Weight 147 aa, 17 kDa
GenBank Accession NumberBC153128
Gene Symbol PRY2
Gene ID (NCBI) 442862
RRIDAB_2879672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRY is Testis-specific PTP-BL-related Y protein which has two isoforms with MW 16 and 6-8 kDa.

Protocols

Product Specific Protocols
WB protocol for PRY antibody 24688-1-APDownload protocol
IHC protocol for PRY antibody 24688-1-APDownload protocol
IF protocol for PRY antibody 24688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols