PPP1R13L Polyclonal antibody

PPP1R13L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 51141-1-AP

Synonyms

IASPP, Inhibitor of ASPP protein, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor

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Validation Data Gallery

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  WB analysis of human heart using 51141-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 51141-1-AP (PPP1R13L antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 51141-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51141-1-AP (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 51141-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51141-1-AP (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51141-1-AP (PPP1R13L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51141-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51141-1-AP (PPP1R13L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human heart tissue, HEK-293 cells, mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

51141-1-AP targets PPP1R13L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R13L fusion protein Ag0635
• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Calculated Molecular Weight: 45 kDa, 89 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC032298
• Gene Symbol: PPP1R13L
• Gene ID (NCBI): 10848
• RRID: AB_2268619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Med; Exome-wide evaluation of rare coding variants using electronic health records identifies new gene-phenotype associations.; Authors - Joseph Park
human	WB,IHC	Adv Sci (Weinh); Chitosan-Gelatin-EGCG Nanoparticle-Meditated LncRNA TMEM44-AS1 Silencing to Activate the P53 Signaling Pathway for the Synergistic Reversal of 5-FU Resistance in Gastric Cancer; Authors - Mi Zhou ; KD Validated