POLL Polyclonal antibody

POLL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29920-1-AP

Synonyms

BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL

---

Validation Data Gallery

• 

  WB analysis using 29920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, HepG2 cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29920-1-AP targets POLL in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLL fusion protein Ag31995
• Full Name: polymerase (DNA directed), lambda
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC068529
• Gene Symbol: POLL
• Gene ID (NCBI): 27343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195).