PDE1B Polyclonal antibody

PDE1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13121-1-AP

Synonyms

63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B

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Validation Data Gallery

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  WB analysis using 13121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13121-1-AP targets PDE1B in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE1B fusion protein Ag3817
• Full Name: phosphodiesterase 1B, calmodulin-dependent
• Calculated Molecular Weight: 536 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC032226
• Gene Symbol: PDE1B
• Gene ID (NCBI): 5153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE1B, also known as PDES1B, belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. PDE1B degrades cAMP and cGMP and regulates various important physiological processes such as proliferation and lipogenesis (PMID: 21960012). PDE1B is enriched in brain tissue. PDE1B gene consists of two isoforms, PDE1B1 (encoding 61 kDa protein with 536 amino acid residues) and PDE1B2 (encoding 59 kDa protein containing 516 amino acid residues).