PCDHA6 Polyclonal antibody

PCDHA6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12853-1-AP

Synonyms

CNR2, CNRN2, CNRS2, CRNR2, PCDH alpha 6, PCDH ALPHA6, PCDHA6, protocadherin alpha 6

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Validation Data Gallery

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  WB analysis of rat brain using 12853-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12853-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12853-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12853-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 12853-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue, human gliomas tissue, rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12853-1-AP targets PCDHA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCDHA6 fusion protein Ag3493
• Full Name: protocadherin alpha 6
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC036674
• Gene Symbol: PCDHA6
• Gene ID (NCBI): 56142
• RRID: AB_10642141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.