CoraLite® Plus 488-conjugated P27; KIP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

P27; KIP1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25614

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1

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Validation Data Gallery

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  IF Staining of mouse testis using CL488-25614

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 P27; KIP1 antibody (CL488-25614) at dilution of 1:200.

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  IF Staining of mouse testis using CL488-25614

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 P27; KIP1 antibody (CL488-25614) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25614 targets P27; KIP1 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P27; KIP1 fusion protein Ag22582
• Full Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Calculated Molecular Weight: 198 aa, 22 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC001971
• Gene Symbol: CDKN1B
• Gene ID (NCBI): 1027
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.