CoraLite® Plus 488-conjugated OXCT1 Monoclonal antibody

OXCT1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1B9

Cat no : CL488-67836

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67836

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-67836, Clone: 1G1B9 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67836

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-67836, Clone:1G1B9) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67836 targets OXCT1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OXCT1 fusion protein Ag24792
• Full Name: 3-oxoacid CoA transferase 1
• Calculated Molecular Weight: 520 aa, 56 kDa
• GenBank Accession Number: BC009001
• Gene Symbol: OXCT1
• Gene ID (NCBI): 5019
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)