hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19842-1-AP

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Validation Data Gallery

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  WB analysis using 19842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19842-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19842-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19842-1-AP

  IP result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 19842-1-AP

  Immunofluorescent analysis of HepG2 cells using 19842-1-AP (hIST1 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse brain tissue, PC-3 cells
• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 4 publications below

Product Information

19842-1-AP targets hIST1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: hIST1 fusion protein Ag13874
• Full Name: KIAA0174
• Calculated Molecular Weight: 364 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000116
• Gene Symbol: KIAA0174
• Gene ID (NCBI): 9798
• RRID: AB_2878612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human		MBio; Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.; Authors - Ekansh Mittal
human,mouse	IF	Cell Rep; Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation; Authors - Marine Gros
human	IF	bioRxiv; IST1 regulates select endosomal recycling pathways; Authors - Amy K Clippinger
human	IF	Traffic; IST1 regulates select recycling pathways; Authors - Amy K Clippinger