NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12E12

Cat no : 68182-1-Ig

Synonyms

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

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Validation Data Gallery

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  WB analysis using 68182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68182-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of human lung cancer using 68182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68182-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: rat brain tissue, U2OS cells, mouse brain tissue, A549 cells, A431 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68182-1-Ig targets NSFL1C in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSFL1C fusion protein Ag8040
• Full Name: NSFL1 (p97) cofactor (p47)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC002801
• Gene Symbol: NSFL1C
• Gene ID (NCBI): 55968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.