NACC1 Polyclonal antibody

NACC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14551-1-AP

Synonyms

BEND8, BTBD14B, NAC 1, NAC1, NACC1

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Validation Data Gallery

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  WB analysis using 14551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14551-1-AP (NACC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14551-1-AP

  IP result of anti-NACC1 (IP:14551-1-AP, 4ug; Detection:14551-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NACC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NACC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14551-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using NACC1 antibody (14551-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: mouse ovary tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14551-1-AP targets NACC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NACC1 fusion protein Ag6096
• Full Name: nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC055396
• Gene Symbol: NACC1
• Gene ID (NCBI): 112939
• RRID: AB_2918036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NACC1, also named as BTBD14B and NAC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). NACC1 has 1 isoform with the molecular mass of 57 kDa. While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549).

Publications

Species	Application	Title
human	WB	Oncol Rep; lncRNA FOXP4‑AS1 predicts poor prognosis and accelerates the progression of mantle cell lymphoma through the miR‑423‑5p/NACC1 pathway.; Authors - Hong-Fang Tao
human	WB	J Clin Transl Hepatol; Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis; Authors - Bin Xu
human	WB	Genes Genomics; ST6GALNAC1 promotes the invasion and migration of breast cancer cells via the EMT pathway; Authors - Yunzhao Luo