MYL6 Polyclonal antibody

MYL6 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18158-1-AP

Synonyms

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

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Validation Data Gallery

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  WB analysis using 18158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18158-1-AP (MYL6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18158-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (18158-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse uterus tissue, rat uterus tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18158-1-AP targets MYL6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYL6 fusion protein Ag12964
• Full Name: myosin, light chain 6, alkali, smooth muscle and non-muscle
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC006781
• Gene Symbol: MYL6
• Gene ID (NCBI): 4637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).