CoraLite®594-conjugated MYL4 Monoclonal antibody

MYL4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F5C1

Cat no : CL594-67533

Synonyms

ALC1, AMLC, GT1, MLC1, MYL4, Myosin light chain 4, PRO1957

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-67533

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 MYL4 antibody (CL594-67533, Clone: 2F5C1 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67533 targets MYL4 in IF applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYL4 fusion protein Ag19244
• Full Name: myosin, light chain 4, alkali; atrial, embryonic
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC030228
• Gene Symbol: MYL4
• Gene ID (NCBI): 4635
• RRID: AB_2920152
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.