MTMR15 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTMR15 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17600-1-AP

Synonyms

FAN1, Fanconi associated nuclease 1, KIAA1018, MTMR15

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Validation Data Gallery

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  WB analysis using 17600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 17600-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17600-1-AP

  IP result of anti-MTMR15 (IP:17600-1-AP, 4ug; Detection:17600-1-AP 1:300) with HeLa cells lysate 2800 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, human spleen tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

17600-1-AP targets MTMR15 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTMR15 fusion protein Ag11651
• Full Name: myotubularin related protein 15
• Calculated Molecular Weight: 60 kDa, 114 kDa
• Observed Molecular Weight: 63-68 kDa, 120-140 kDa
• GenBank Accession Number: BC047882
• Gene Symbol: MTMR15
• Gene ID (NCBI): 22909
• RRID: AB_10598016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu
human	WB,IP	Genes Cells; MLH1-mediated recruitment of FAN1 to chromatin for the induction of apoptosis triggered by O6 -methylguanine.; Authors - Mihoko Rikitake ; KD Validated
human	WB	Cell Rep; HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.; Authors - Shaozu Fu