MTF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTF2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, chIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 16208-1-AP

Synonyms

hPCl2, M96, MTF2, PCL2, Polycomb like protein 2, RP5 976O13.1

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Validation Data Gallery

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  WB analysis of HepG2 using 16208-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ES cells and 293 cells using 16208-1-AP

  WB result of anti-MTF2 (16208-1-AP) from Kevin Corbit.

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  WB analysis using 16208-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16208-1-AP (MTF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 16208-1-AP

  IP result of anti-MTF2 (IP:16208-1-AP, 4ug; Detection:16208-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16208-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16208-1-AP (MTF2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse ES cells using 16208-1-AP

  IF result of mouse ES cell From Kevin Corbit.

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, mouse ES cells and 293 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse ES cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Product Information

16208-1-AP targets MTF2 in WB, IP, IF, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTF2 fusion protein Ag9182
• Full Name: metal response element binding transcription factor 2
• Calculated Molecular Weight: 536 aa, 61 kDa
• Observed Molecular Weight: 67 kDa, 60 kDa, 60-61 kDa
• GenBank Accession Number: BC010013
• Gene Symbol: MTF2
• Gene ID (NCBI): 22823
• RRID: AB_2147370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTF2, also named as Metal-response element-binding transcription factor 2, is a 593 amino acid protein. It exists several isoforms and the range of the molecular weight of isoforms are about 55-60 kDa and 29 kDa. MTF2 contains 2 PHD-type zinc fingers and belongs to the Polycomblike family. MTF2 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity and regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation.

Publications

Species	Application	Title
mouse	WB	Nature; Polycomb-like proteins link the PRC2 complex to CpG islands.; Authors - Haojie Li ; KO Validated
mouse	WB	Nat Genet; MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.; Authors - Matteo Perino
human	WB	Nat Genet; RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells.; Authors - Yicheng Long
human	WB	Nat Cell Biol; Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes; Authors - Christina E Sparbier
mouse	WB	Nat Struct Mol Biol; G-tract RNA removes Polycomb repressive complex 2 from genes.; Authors - Manuel Beltran
human	WB,ChIP	Mol Cell; Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.; Authors - Daniel T Youmans