MRPS5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MRPS5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16428-1-AP

Synonyms

MRP S5, MRPS5, S5mt

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Validation Data Gallery

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  WB analysis using 16428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16428-1-AP (MRPS5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16428-1-AP

  WB result of MRPS5 antibody (16428-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS5 transfected HeLa cells.

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  IHC staining of human kidney using 16428-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16428-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 16428-1-AP is MRPS5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPS5 fusion protein Ag9672
• Full Name: mitochondrial ribosomal protein S5
• Calculated Molecular Weight: 430 aa, 48 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC014172
• Gene Symbol: MRPS5
• Gene ID (NCBI): 64969
• RRID: AB_2878258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Transl Oncol; Porphyromonas gingivalis promotes malignancy and chemo-resistance via GSK3β-mediated mitochondrial oxidative phosphorylation in human esophageal squamous cell carcinoma; Authors - Yiwen Liu