MRPL53 Polyclonal antibody

MRPL53 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16142-1-AP

Synonyms

L53mt, MRP L53, MRPL53

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Validation Data Gallery

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  WB analysis of A549 using 16142-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16142-1-AP (MRPL53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 16142-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 16142-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16142-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16142-1-AP (MRPL53 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16142-1-AP targets MRPL53 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL53 fusion protein Ag9210
• Full Name: mitochondrial ribosomal protein L53
• Calculated Molecular Weight: 112 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC012163
• Gene Symbol: MRPL53
• Gene ID (NCBI): 116540
• RRID: AB_2878223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.